北京辰辉创聚生物技术有限公司主要开发和生产重组蛋白产品。经过多年的发展和成长,我们目前拥有超过 10,000 种蛋白产品。此外,我们还扩展了一系列相关产品,例如天然蛋白、细胞/组织裂解物、单/多克隆抗体和ELISA检测试剂盒。为了满足客户的需求,我司不断推出热门类别,例如细胞因子、标记蛋白、全长蛋白、病毒相关蛋白、生物标志物、CD抗原等。此外,我们还根据行业客户的需求提供 GMP级别蛋白。
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Actin | DNA-Damage Protein | Methylmalonic Aciduria |
Adaptor-Related Protein Complex | Dynactin | MHC class I chain-related gene |
ADP-Ribosylation Factor | Dynein Light Chain | Microfibrillar Associated Protein |
Ag85 | Dysbindin | Microtubule-Associated Protein |
Albumin | Ectodysplasin | Mitochondrial Ribosomal Protein |
Allergy | Elongator Acetyltransferase Complex | Mitochondrial Transcription Factor |
Alpha-2-HS-Glycoprotein | Endoplasmic Reticulum Protein | MOB1, Mps One Binder Kinase Activator |
Anaplasma | Endothelial Cell-Specific Molecule 1 | Mortality Factor |
Angiogenin | EPH Receptor | Myelin Basic Protein |
Ankyrin Repeat Domain | Ephrin | Myelin Oligodendrocyte Glycoprotein |
Annexin | ERCC | Myoglobin |
Anterior Gradient Protein | Eukaryotic Translation Initiation Factor | Myosin Light Chain |
ASF1 Anti-Silencing Function 1 | Exosome Component | Myxovirus |
ATP Synthase Mitochondrial | FABP | NANOG |
ATPase | Family with Sequence Similarity | NCK Adaptor Protein |
Autophagy Related | F-Box Protein | NECTIN |
B Cell Lymphoma | Ferritin | Nescient Helix Loop Helix |
B9 Protein | Fibrinogen | Neuronal Calcium Sensor |
Babesia Microti | Fibronectin Type III Domain Containing | Neutrophil Cytosolic Factor |
Baculoviral IAP Repeat-Containing | Folate Receptor | NFKB Inhibitor |
Bartonella H. | Four And A Half LIM | NHP2 |
Basic Transcription Factor | Fragile Histidine Triad | N-Myc Downstream Regulated |
BATF | G Antigen | Non-Metastatic Cells |
Bax | GABA(A) Receptor-Associated Protein | NPM |
Beta 2 Microglobulin | Gastrokine | NTF2-like Export Factor |
BID | GDP Dissociation Inhibitor | Nucleobindin |
Biglycan | General Transcription Factor | Nucleopurin |
Bridging Integrator | GIPC PDZ Domain | Nucleosome Assembly Protein |
Bromodomain Containing | Gliadin | Orosomucoid |
Cadherin | Glycophorin | Toll Like Receptor |
Calbindin | Glycoprotein Nmb | Outer Membrane Protein |
Calcium Binding Protein | Glypican | p53 |
Calcyphosine | Gremlin | Paired Box |
Calmodulin | GTPase IMAP Family | Parkinson Disease Protein |
Calpain | GTP-Binding Protein | Parvalbumin |
Calponin | Guanine Nucleotide Binding Protein | PCNA |
Calreticulin | Hairy and Enhancer of Split | PDZ Domain Containing |
Calumenin | Haptoglobin | Pentraxin |
Candida Albicans | Heat Shock Protein | Peroxisomal Biogenesis Factor |
Canopy FGF Signaling Regulator | Hematological And Neurological Expressed | PHD Finger Protein |
Capping Protein | Hemoglobin | Phosducin-Like |
Caspase Recruitment Domain Family | Hemopexin | Phosphatase and Tensin |
CCR4-NOT Transcription Complex | High-Mobility Group | Phospholipid Scramblase |
CEA | HINT | PIH1 Domain Containing |
CEBP | HLA | Pim Oncogene |
Cell Division Cycle | HNRNP | PITPN |
Cellular Retinoic Acid Binding Protein | Homeobox | Polymerase (RNA) (DNA directed) Polypeptide |
Centrin | Homer Homolog | POU Class |
Centromere Protein | Hypoxia-Inducible Factor | Prefoldin |
Charged Multivesicular Body Protein | Ig Heavy Chain Constant Region | Pregnancy Specific Beta-1-Glycoprotein |
Chloride Intracellular Channel | IMPAD1 | Prion Protein |
Chromatin Modifying Protein | Inhibitor of DNA Binding | Processing Of Precursor |
Chromobox | Inhibitor of Growth Family | Profilin |
Chromogranin | Integrin | Programmed Cell Death |
Chromosome Open Reading Frame | Intercellular Adhesion Molecule | Prohibitin |
Clathrin | Junctional Adhesion Molecule | Proprotein Convertase Subtilisin/Kexin |
Coagulation Factors | Karyopherin | Protein C-Ets |
Cofilin | KCTD | Protein Phosphatase |
Coiled-Coil Domain | Killer Cell | Protein-A, A/G & G |
Collagen | Killer Cell Lectin-like Receptor | Prothymosin |
COMM Domain Containing | Kirsten Rat Sarcoma Viral Oncogene | RAD51 |
Complement Component | Kruppel-Like Factor | RAN Binding Protein |
COP9 Signalosome | Lactoferrin | Ras-Related C3 Botulinum Toxin Substrate |
C-Reactive Protein | LBP | RASSF |
Crystallin | Left-Right Determination Factor | Receptor Activity-Modifying Protein |
C-type Lectin Domain | Leukocyte Cell Derived Chemotaxin | Regenerating Islet-Derived |
CUE Domain Containing | Leukocyte-Associated Ig-Like Receptor | Regulator of Calcineurin |
Cystatin | LIN Protein | Regulator of G-Protein Signaling |
Cysteine-Rich | Listeriolysin | Related RAS Viral (r-ras) Oncogene |
Cysteine-Rich Secretory Protein | LRG1 | Relaxin |
Cytochrome | Lymphocyte Antigen | Renin |
Cytohesin | Lysosomal-Associated Membrane Protein | Reticulocalbin |
Cytokeratin | MAD2 | Retinoblastoma |
DCUN1D | MAF | Retinoic Acid Early Transcript |
DEAD Box Protein | Mago-Nashi Homolog | Retinoic Acid Receptor Responder |
Decorin | Maltose Binding Protein | Rho Family GTPase |
Density Lipoprotein | Mediator Complex | Rho GDP Dissociation Inhibitor |
Developmental Pluripotency Associated | Melanoma Antigen Family A | Ribosomal Protein |
Dickkopf-Related Protein | Member RAS Oncogene Family | Ring Finger Protein |
DiGeorge Syndrome Critical Region | Mesoderm Development Candidate | RNA Binding Motif Protein |
Zinc Finger | Met Proto-Oncogene | R-Spondin |
WAP Four-Disulfide Core Domain | Y. Enterocolitica | Trafficking Protein Particle Complex |
RWD Domain Containing | Sorting Nexin | Transcription Elongation Factor |
Sclerostin | SPARC | Transferrin |
SDHAF | SPSB | Transforming Growth Factor Beta Induced |
Secretagogin | sRAGE | Transgelin |
Secreted Frizzled-Related Protein | SRY (Sex Determining Region Y)-Box | Translocase Of Outer Mitochondrial Membrane |
Secretoglobin | Stathmin | Triggering Receptor Expressed on Myeloid Cells |
Secretogranin | STIP | Tripartite Motif |
Selectin | Streptavidin Proteins | Tropomyosin |
Selenoprotein | Superoxide Dismutase | Troponin |
Septin | Surfeit | TROVE Domain Family |
Serglycin | Synaptobrevin | Trypsin |
Serine Peptidase Inhibitor | Synaptosomal Associated Protein | TTC |
Serpin | Synaptotagmin | Tubulin Folding Cofactor |
SERTA Domain Containing | Syndecan | Tubulin Gamma |
SH2 Domain | Synovial Sarcoma, X Breakpoint | U6 Small Nuclear RNA |
SH3 Domain | Syntaxin | Ubiquinol-Cytochrome C Reductase |
SIGLEC | Synuclein | Ubiquitin |
Signal Recognition Particle | Tachykinin | UCHL1 |
Signal Sequence Receptor | TAR DNA | UL16 binding protein |
Signal-Regulatory Protein | TBC1 Domain Family | Uroplakin |
Single-Stranded DNA Binding Protein | TCL | Vacuolar Protein Sorting |
Sirtuin | THAP Domain | Vascular cell adhesion molecule |
SIX Homeobox | Thioredoxin | V-crk Sarcoma Virus CT10 |
SLAM Family | TIGAR | Vimentin |
SMAD | Tissue Factor Pathway Inhibitor | Visinin-Like Protein |
SNRP | TNF receptor-Associated Factor | V-ral Simian Leukemia Viral Oncogene |
Solute Carrier Family |
重组蛋白是通过基因工程技术将不同来源的DNA序列组合在一起,构建出新的DNA分子,并通过这些DNA分子的表达来生产特定蛋白。通过重组DNA技术,可以大量生产天然型或改良版的人的和哺乳动物的蛋白。重组蛋白通常来源于克隆的DNA序列编码,这些序列通常是编码具有已知功能的酶或蛋白质。
重组蛋白的生产依赖于基因工程技术,也称为基因拼接或重组DNA技术。通过将人类、动物或植物的基因导入细菌、哺乳动物(CHO, HEK293, sf9等细胞)或酵母细胞的遗传物质中,这些微生物便可以作为“工厂”,生产用于医疗、学术和科研用途的蛋白。
载体是一种操纵DNA的工具,可以被视为从克隆到其中的特定DNA序列生产蛋白质的“运输工具”。重组蛋白是通过人工构建的DNA生成的蛋白质,这些来自于两个或更多来源的DNA,被整合为一个重组分子。首先,使用限制性内切酶处理DNA,其切口末端有一段单链DNA,被称为“粘性末端”,因为它们能够与包含互补粘性末端的DNA分子进行碱基互补配对。随后,DNA连接酶将这两条DNA链共价连接,形成一个重组DNA分子。
重组DNA分子需要经过多次复制,以提供用于分析和测序的材料。产生多个相同的重组DNA分子叫做克隆。克隆是在体外通过聚合酶链式反应(PCR)进行扩增的,或者在单细胞微生物(如大肠杆菌)、单细胞真核生物(如酵母)或哺乳动物细胞培养中进行体内克隆。重组DNA必须以可以复制和表达的形式被细胞吸收。这是通过将DNA整合到载体中来实现的。多种病毒(包括细菌病毒和哺乳动物细胞病毒)都可以作为载体。
重组DNA也被称为“嵌合体”,当结合两种或更多不同来源的DNA链时,重组DNA的构建可以通过三种不同的方法进行:1. 转化法,2. 噬菌体转染法,3. 酵母、植物和哺乳动物转化法。在转化法中,首先需要选择要插入载体中的DNA片段,使用限制性酶切割DNA,并用DNA连接酶将DNA插入到载体中。插入的DNA包含可选择标记,可用于识别重组分子。使用抗生素标记是为了在暴露于某种抗生素时导致不含载体的宿主细胞死亡。转化是指将载体插入宿主细胞。宿主细胞被处理以便吸收外源DNA。可选择的标记用于抗生素抗性、颜色变化或任何其他可以区分转化和非转化宿主的特征。酵母、植物和哺乳动物转化法是通过显微注射将DNA注入到细胞核中。噬菌体转染过程与转化类似,但使用的是噬菌体λ或M13代替细菌。这些噬菌体产生含有重组蛋白的噬菌斑,可以通过各种选择方法将其与非重组蛋白区分开来。
只有当表达基因被添加时,宿主才会生产大量重组蛋白。蛋白质的表达依赖于围绕目标DNA的基因,这些基因集合充当信号,为细胞转录和翻译目标DNA提供指令。这些信号包括启动子、核糖体结合位点和终止子。重组DNA被插入到表达载体中,这些载体包含了启动子、核糖体结合位点和终止子。在原核系统中,启动子、核糖体结合位点和终止子必须来自同一宿主,因为细菌通常无法识别人类的启动子和终止子信号。指定的基因不能含有人体内含子,因为细菌无法识别内含子,这会导致转录提前终止,并且重组蛋白可能无法正确加工、折叠,甚至可能被降解。
蛋白质的纯化和表达过程有时会非常复杂且费时,因此除了特定的DNA序列外,还使用额外的标签,以促进重组蛋白的纯化和表达。肽序列可以作为延伸序列标签添加到N端。重组蛋白的常见标签包括His标签、Flag标签、GST标签、His标签、HA标签、Myc标签和GFP标签等。这些标签在重组蛋白表达和纯化中发挥着重要作用。蛋白标签通常放置在载体中,以便将其设计为特定蛋白酶的接触点。因此,在重组蛋白表达并从细菌中提取后,可以使用特定的标签来纯化蛋白,然后从目标蛋白中去除,最终得到几乎天然的最终蛋白产品。